Mouse Granzyme B ELISA Kit PicoKine®

Mfr.No.

EK1115

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.032	0.081	0.163	0.292	0.497	0.904	1.478	1.992

Description

Mouse Granzyme B ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Gzmb in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Granzyme B (G,H);3.4.21.79;CTLA-1;Cytotoxic cell protease 1;CCP1;Fragmentin-2;Gzmb;Ctla-1, Ctla1;

Molecular Weight

27470 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: I21-S247

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Granzyme B

Subcellular Localization

Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	31	151	486	31	149	483
Standard deviation	1.39	11.77	25.75	1.89	14	29.94
CV (%)	4.5%	7.8%	5.3%	6.1%	9.4%	6.2%

Protein Function

This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis (By similarity). .

Background

Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are thought to protect their host by inducing apoptosis of cells that bear on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	31	32	30	33	31	1.11	3.5%
Sample 2	151	172	146	157	156	9.75	6.2%
Sample 3	486	437	481	454	464	20	4.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	14939
Gene Name	Gzmb
UniProt ID	P04187
Protein Name	Granzyme B(G,H)

Applications	ELISA
Research Area	Adaptive Immunity,Apoptosis,Cell Biology,Immunology,Innate Immunity,NK Cells,T Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001276	EK1115	96 wells/kit	$499		Add to Cart

Mouse Granzyme B ELISA Kit PicoKine®

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