Mouse GPNMB/Osteoactivin ELISA Kit PicoKine®

Mfr.No.

EK1331

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10,000
O.D.	0.007	0.087	0.143	0.276	0.478	0.783	1.395	2.006

Description

Mouse GPNMB/Osteoactivin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Gpnmb in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Transmembrane glycoprotein NMB;DC-HIL;Dendritic cell-associated transmembrane protein;Osteoactivin;Gpnmb;Dchil, Hgfin, Nmb;

Molecular Weight

63676 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: K23-N502

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Osteoactivin

Subcellular Localization

Cell membrane ; Single-pass type I membrane protein . Endomembrane system ; Single-pass type I membrane protein . Cytoplasmic vesicle membrane ; Single-pass type I membrane protein . Melanosome . Vesicular, endosomal like structures.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	265	1462	4620	283	1371	4849
Standard deviation	13.78	73.1	258.72	19.24	80.88	353.97
CV (%)	5.2%	5%	5.6%	6.8%	5.9%	7.3%

Protein Function

Could be a melanogenic enzyme. .

Background

Transmembrane glycoprotein NMB is a protein that is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis (soft callus) and osteogenesis (hard callus) compared to the intact femurs that is why Osteoactivin (OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	265	290	285	290	282	10.3	3.6%
Sample 2	1462	1602	1474	1607	1536	68.4	4.4%
Sample 3	4620	4438	4374	4575	4501	99.66	2.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	93695
Gene Name	GPNMB
UniProt ID	Q99P91
Protein Name	Transmembrane glycoprotein NMB
Tissue Specificity	In early development, mRNA is detected at high levels in the outer layer of the retina. Later in development expression gets restricted to the retinal pigment epithelium and iris. Overexpressed in mammary cancer cells possessing a strong bone metastatic phenotype. .
Sequence	Belongs to the PMEL/NMB family.

Applications	ELISA
Research Area	Cancer,Immunology,Innate Immunity,Macrophage/Inflammation,Tumor Immunology,Tumor-Associated Antigens

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000929	EK1331	96 wells/kit	$499		Add to Cart

Mouse GPNMB/Osteoactivin ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse GPNMB/Osteoactivin ELISA Kit PicoKine®

Rate it now..!!