Mouse Glycated hemoglobin A1c,GHbA1c ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08141m

Sensitivity

0.39 ug/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Competitive

Recovery

The recovery of sheep AVP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	92	86-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep AVP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-94
1:2	Average %	101
	Range %	95-105
1:4	Average %	92
	Range %	87-95
1:8	Average %	98
	Range %	94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	GHbA1c

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Vitamin D3 supplementation of a high fat high sugar diet ameliorates prediabetic phenotype in female LDLR Ramiar Kheder.et al,J BONE MINER RES,Mar 13 Glyoxalase 1 Attenuates the Effects of Chronic Hyperglycemia on Explant-Derived Cardiac Stem Cells Villanueva.et al,/,/

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