Mouse Glutamate [NMDA] receptor subunit epsilon-2(GRIN2B) ELISA kit

Mouse Glutamate [NMDA] receptor subunit epsilon-2(GRIN2B) ELISA kit

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Mfr.No.

CSB-EL009913MO

Sensitivity

7.8 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

glutamate receptor, ionotropic, N-methyl D-aspartate 2B, MGC142178, MGC142180, NMDAR2B, NR2B, hNR3, N-methyl-D-aspartate receptor subunit 2B, glutamate receptor subunit epsilon-2

Recovery

The recovery of human MGLL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-91
EDTA plasma (n=4)	94	91-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MGLL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	104
	Range %	101-107
1:4	Average %	97
	Range %	92-102
1:8	Average %	98
	Range %	93-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2500	2.524	2.537	2.531	2.370
1250	1.661	1.687	1.674	1.513
625	1.076	1.082	1.079	0.918
312.5	0.734	0.704	0.719	0.558
156.25	0.511	0.504	0.508	0.347
78.13	0.375	0.392	0.384	0.223
39.06	0.302	0.298	0.300	0.139
0	0.165	0.157	0.161

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q01097

Applications	ELISA
Research Area	GRIN2B

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505803	CSB-EL009913MO	96T	$695	Add to Cart
BHE10505803	CSB-EL009913MO	5x96T	$3336	Add to Cart
BHE10505803	CSB-EL009913MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.