Mouse Glucose Regulated Protein 78(GRP78)Elisa kit

Mouse Glucose Regulated Protein 78(GRP78)Elisa kit

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Mfr.No.

CSB-E17690m

Alternative Names

Glucose Regulated Protein 78(GRP78)Elisa kit, BIP, FLJ26106, GRP78, MIF2, Heat-shock 70kD protein-5 (glucose-regulated protein, 78kD), heat shock 70kD protein 5 (glucose-regulated protein, 78kD), heat shock 70kDa protein 5

Reactivity

Mouse

Detection Range

0.45 ng/mL-30 ng/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human DOPEY2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	102-106
EDTA plasma (n=4)	93	90-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DOPEY2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-97
1:2	Average %	97
	Range %	92-103
1:4	Average %	85
	Range %	81-89
1:8	Average %	89
	Range %	83-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.313	2.451	2.382	2.335
640	1.754	1.802	1.778	1.731
320	1.012	1.032	1.022	0.975
80	0.345	0.355	0.350	0.303
32	0.173	0.174	0.174	0.127
0	0.045	0.048	0.047

Species

Mus musculus

Sensitivity

0.11 ng/mL

Limit Of Detection

450 nm

UniProt ID	P20029
Antigen	Glucose Regulated Protein 78(GRP78)Elisa kit

Applications

ELISA

Research Area

GRP78

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505800	CSB-E17690m	96T	$790	Add to Cart
BHE10505800	CSB-E17690m	5x96T	$3792	Add to Cart
BHE10505800	CSB-E17690m	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.