Matrices listed below were spiked with certain level of GLS and the recovery rates were calculated by comparing the measured value to the expected amount of GLS in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
95-105
100
EDTA plasma(n=5)
85-102
93
heparin plasma(n=5)
86-100
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GLS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-102%
88-104%
85-105%
EDTA plasma(n=5)
87-96%
83-96%
89-94%
heparin plasma(n=5)
80-91%
80-95%
82-99%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
D3Z7P3
Applications
ELISA
Applications Notes
GLS ELISA Kit allows for the in vitro quantitative determination of GLS concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse GLS(Glutaminase kidney isoform, mitochondrial) ELISA Kit