Mouse Glia-derived nexin(SERPINE2) ELISA kit

Mouse Glia-derived nexin(SERPINE2) ELISA kit

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Mfr.No.

CSB-EL021082MO

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2, GDN, PI7, PN1, PNI, OTTHUMP00000203766, glial-derived neurite promoting factor, glial-derived nexin 1, plasminogen activator inhibitor type 1, member 2, protease inhibitor 7 (protease nexin I), serine (o

Recovery

The recovery of human IGHG3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-94
EDTA plasma (n=4)	95	90-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IGHG3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	99
	Range %	93-106
1:2000	Average %	90
	Range %	85-95
1:4000	Average %	87
	Range %	82-91
1:8000	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
2000	0.173	0.172	0.1725
667	0.259	0.246	0.2525
222	0.367	0.344	0.3555
74	0.488	0.459	0.4735
25	0.633	0.624	0.6285
8.2	0.789	0.855	0.822
2.7	1.029	1.033	1.031
0	1.697	1.748	1.7225

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q07235

Applications	ELISA
Research Area	SERPINE2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505787	CSB-EL021082MO	96T	$695	Add to Cart
BHE10505787	CSB-EL021082MO	5x96T	$3336	Add to Cart
BHE10505787	CSB-EL021082MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.