Matrices listed below were spiked with certain level of Gfap and the recovery rates were calculated by comparing the measured value to the expected amount of Gfap in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
96
EDTA plasma(n=5)
87-99
92
heparin plasma(n=5)
93-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gfap and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Gfap ELISA Kit allows for the in vitro quantitative determination of Gfap concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Gfap(Glial fibrillary acidic protein) ELISA Kit