Matrices listed below were spiked with certain level of Gapdh and the recovery rates were calculated by comparing the measured value to the expected amount of Gapdh in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
96
EDTA plasma(n=5)
89-97
93
heparin plasma(n=5)
85-101
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gapdh and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Gapdh/Peptidyl-cysteine S-nitrosylase GAPDH/GAPD/G3PD/G3PDH/aging-associated gene 9 protein
Notes
For Research Use Only
UniProt ID
P16858
Antigen
GAPDH
Applications
ELISA
Applications Notes
Gapdh ELISA Kit allows for the in vitro quantitative determination of Gapdh concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Gapdh (Glyceraldehyde-3-phosphate dehydrogenase) ELISA Kit