Mouse gamma-Secretase ELISA kit

Mouse gamma-Secretase ELISA kit

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Mfr.No.

CSB-E12142m

Sensitivity

1.95 pg/mL

Detection Range

7.8 pg/mL-500 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

anterior pharynx defective 1 homolog B (C. elegans), APH-1B, DKFZp564D0372, FLJ33115, PRO1328, PSFL, TAAV688, anterior pharynx defective 1B-like, presenilin stabilization factor-like, gamma secretase

Recovery

The recovery of human C1QTNF6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-96
EDTA plasma (n=4)	90	82-93

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C1QTNF6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	105
	Range %	99-108
1:4	Average %	90
	Range %	84-94
1:8	Average %	87
	Range %	82-91

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.778	2.662	2.720	2.600
10	1.926	1.905	1.916	1.796
5	1.315	1.342	1.329	1.209
2.5	0.703	0.734	0.719	0.599
1.25	0.390	0.381	0.386	0.266
0.625	0.295	0.288	0.292	0.172
0.312	0.203	0.209	0.206	0.086
0	0.119	0.121	0.120

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q8C7N7

Applications	ELISA
Research Area	APH1B

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506961	CSB-E12142m	96T	$790	Add to Cart
BHE10506961	CSB-E12142m	5x96T	$3792	Add to Cart
BHE10506961	CSB-E12142m	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.