Mouse Galectin-3-binding protein(LGALS3BP) ELISA kit

Mouse Galectin-3-binding protein(LGALS3BP) ELISA kit

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Mfr.No.

CSB-EL012888MO

Sensitivity

0.09 ng/mL

Detection Range

0.39 ng/mL-25 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

lectin, galactoside-binding, soluble, 3 binding protein, 90K, BTBD17B, MAC-2-BP, L3 antigen, Mac-2-binding protein, galectin 3 binding protein, serum protein 90K

Recovery

The recovery of human ITIH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	88-95
EDTA plasma (n=4)	102	97-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ITIH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	91
	Range %	86-94
1:10	Average %	97
	Range %	90-104
1:20	Average %	101
	Range %	96-107
1:40	Average %	89
	Range %	84-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average	Corrected
200	2.439	2.555	2.497	2.312
100	1.867	1.728	1.798	1.613
50	1.221	1.265	1.243	1.058
25	0.751	0.757	0.754	0.569
12.5	0.412	0.422	0.417	0.232
6.25	0.318	0.336	0.327	0.142
3.12	0.272	0.281	0.277	0.092
0	0.183	0.186	0.185

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q07797

Applications	ELISA
Research Area	LGALS3BP

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505765	CSB-EL012888MO	96T	$695	Add to Cart
BHE10505765	CSB-EL012888MO	5x96T	$3336	Add to Cart
BHE10505765	CSB-EL012888MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.