Mouse GAD1(Glutamate Decarboxylase 1, Brain) ELISA Kit

Mfr.No.

ELK7113

Sensitivity

0.31 ng/mL

Detection Range

0.79-50 ng/mL

Reactivity

Mouse

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

GAD67; SCP; 67 kDa glutamic acid decarboxylase; Glutamate decarboxylase 67 kDa isoform

Recovery

Matrices listed below were spiked with certain level of recombinant GAD1 and the recovery rates were calculated by comparing the measured value to the expected amount of GAD1 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-95%	89%
EDTA plasma(n=5)	85-97%	91%
Heparin plasma(n=5)	81-95%	88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GAD1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-94%	92-101%	87-96%	82-90%
EDTA plasma(n=5)	81-94%	93-107%	93-102%	91-105%
Heparin plasma(n=5)	89-97%	93-102%	81-96%	93-106%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
50.00	1.933	1.844
25.00	1.695	1.606
12.50	1.215	1.126
6.25	0.921	0.832
3.13	0.519	0.430
1.57	0.335	0.246
0.79	0.179	0.090
0.00	0.089	0.000

Sample Type

Tissue homogenates, Other biological fluids

Limit Of Detection

50 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GAD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GAD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GAD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GAD1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	P48318

Applications	ELISA
Research Area	Enzyme & Kinase;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15206082	ELK7113	48T	$320		Add to Cart
BHE15206082	ELK7113	96T	$458		Add to Cart

Mouse GAD1(Glutamate Decarboxylase 1, Brain) ELISA Kit

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