Matrices listed below were spiked with certain level of GAD-Ab-IgM and the recovery rates were calculated by comparing the measured value to the expected amount of GAD-Ab-IgM in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-103
97
EDTA plasma(n=5)
85-101
93
heparin plasma(n=5)
85-105
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GAD-Ab-IgM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
GAD-Ab-IgM ELISA Kit allows for the in vitro quantitative determination of GAD-Ab-IgM concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse GAD-Ab-IgM(Glutamic Acid Decarboxylase Autoantibody IgM) ELISA Kit