Matrices listed below were spiked with certain level of Gaa and the recovery rates were calculated by comparing the measured value to the expected amount of Gaa in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
93
EDTA plasma(n=5)
88-99
94
heparin plasma(n=5)
92-98
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gaa and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Gaa ELISA Kit allows for the in vitro quantitative determination of Gaa concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Gaa(Lysosomal alpha-glucosidase) ELISA Kit