Mouse Filaggrin(FLG) ELISA kit

Mfr.No.

CSB-EL008712MO

Alternative Names

filaggrin, ATOD2, epidermal filaggrin

Reactivity

Mouse

Detection Range

3.12 pg/mL-200 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human MMP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-105
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	107
	Range %	102-110
1:4	Average %	95
	Range %	90-101
1:8	Average %	89
	Range %	86-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

0.78 pg/mL

Limit Of Detection

450 nm

UniProt ID	P11088
Antigen	Filaggrin

Applications

ELISA

Research Area

FLG

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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