Mouse Fibronectin Type 3 Domain-containing Protein 5

Mfr.No.

E1278Mo

Sensitivity

1.04ng/L

Detection Range

2-700ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Fibronectin type Ⅲ domain-containing protein 5 (also known as FNDC5) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

1500001L03Rik;AI836596;C87088;Fibronectin type III domain-containing protein 5;Fibronectin type III repeat-containing protein 2;FNDC5;Irisin);PeP;Peroxisomal protein;Pxp

Molecular Weight

23321

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1278Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	141.18	7.22	5.12
Intra-Assay	2	18	291.75	7.74	2.65
Intra-Assay	3	18	47.4	2.62	5.52

Background

Fibronectin type III domain-containing protein 5, the precursor of irisin, is a type I transmembrane glycoprotein that is encoded by the FNDC5 gene. Irisin is a cleaved version of FNDC5, named after the Greek messenger goddess Iris. Fibronectin domain-containing protein 5 is a membrane protein comprising a short cytoplasmic domain, a transmembrane segment, and an ectodomain consisting of a ~100 kDa fibronectin type III (FNIII) domain.

Protocol

These standard curves of E1278Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
400ng/L	2.313	2.366	2.298
	2.42
200ng/L	1.294	1.368	1.3
	1.443
100ng/L	0.762	0.75	0.682
	0.737
50ng/L	0.401	0.477	0.409
	0.554
25ng/L	0.243	0.264	0.196
	0.285
0ng/L	0.077	0.068	0
	0.059

Gene ID	384061
UniProt ID	Q8K4Z2

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109143	E1278Mo	48T	$320		Add to Cart
BHE12109143	E1278Mo	96T	$458		Add to Cart

Mouse Fibronectin Type 3 Domain-containing Protein 5 - FNDC5 ELISA Kit

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