Mouse Fibroblast Growth Factor-23

Mfr.No.

E1186Mo

Description

This sandwich kit is for the accurate quantitative detection of Fibroblast growth factor-23 (also known as FGF-23) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

FGF 23;FGF23;FGF-23;Fibroblast growth factor 23

Reactivity

Mouse

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

27758

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.39ng/L

Background

Regulator of phosphate homeostasis (By similarity). Inhibits renal tubular phosphate transport by reducing SLC34A1 levels (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism (By similarity). Negatively regulates osteoblasts differentiation and matrix mineralization (By similarity). Upregulates EGR1 expression in the presence of KL.

Gene ID	64654
UniProt ID	Q9EPC2
Antigen	FGF23

Research Area

Signal Transduction

Protocol

These standard curves of E1186Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.297	2.235	2.211
	2.173
800ng/L	1.223	1.335	1.311
	1.446
400ng/L	0.784	0.691	0.667
	0.599
200ng/L	0.408	0.375	0.351
	0.343
100ng/L	0.176	0.171	0.147
	0.166
0ng/L	0.028	0.024	0
	0.02

Precision

We measured random samples of E1186Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	282.8	14.64	5.2
Intra-Assay	2	18	607.4	29.02	4.8
Intra-Assay	3	18	900.7	25.8	2.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109051	E1186Mo	48T	$320		Add to Cart
BHE12109051	E1186Mo	96T	$458		Add to Cart

Mouse Fibroblast Growth Factor-23 - FGF-23 ELISA Kit

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