Mouse Fibrillin-1 - FBN1 ELISA Kit

Mfr.No.

E1431Mo

Sensitivity

0.013ng/ml

Detection Range

0.2-60ng/ml

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Mouse Cathepsin L (also known as Cath-L) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Basement-membrane protein 90;BM-90;FBLN 1;FBLN1;FIBL-1;Fibulin-1

Molecular Weight

78033

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1431Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	20.4	0.55	2.7
Intra-Assay	2	18	8.9	0.43	4.9
Intra-Assay	3	18	5	0.36	7.2

Background

Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes.

Protocol

These standard curves of E1431Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.435	2.326	2.275
	2.216
16ng/ml	1.4	1.367	1.317
	1.334
8ng/ml	0.774	0.749	0.698
	0.723
4ng/ml	0.505	0.462	0.411
	0.419
2ng/ml	0.254	0.284	0.234
	0.315
0ng/ml	0.042	0.051	0
	0.059

Gene ID	14114
UniProt ID	Q08879

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109293	E1431Mo	48T	$320		Add to Cart
BHE12109293	E1431Mo	96T	$458		Add to Cart

Mouse Fibrillin-1 - FBN1 ELISA Kit

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