Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Fgf21 and the recovery rates were calculated by comparing the measured value to the expected amount of Fgf21 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-103
97
EDTA plasma(n=5)
85-105
97
heparin plasma(n=5)
88-104
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fgf21 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Fgf21 ELISA Kit allows for the in vitro quantitative determination of Fgf21 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Fgf21 (Fibroblast growth factor 21) ELISA Kit