Mouse Fetuin A ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14042m

Sensitivity

1.56 ng/mL

Detection Range

6.25 ng/mL-400 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Sample Volume

50-100ul

Alternative Names

alpha-2-HS-glycoprotein, A2HS, AHS, FETUA, HSGA, fetuin-A

Recovery

The recovery of human ?-GST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	88	84-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ?-GST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	98-109
1:2	Average %	86
	Range %	81-90
1:4	Average %	93
	Range %	89-98
1:8	Average %	90
	Range %	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P29699
Tissue Specificity	Liver is the major site of synthesis, but fetuin is also expressed in limb buds and other extrahepatic tissues during development.

Applications	ELISA
Research Area	AHSG

Storage	Store at 2-8°C. Please refer to protocol.

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