Mouse FceRII(Receptor II For The Fc Region Of Immunoglobulin E) ELISA Kit

Mfr.No.

ELK3208

Sensitivity

0.66 pg/mL

Detection Range

1.57-100 pg/mL

Reactivity

Mouse

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

CD23; FCER2; BLAST-2; CLEC4J; FCE2; IGEBF; C-type lectin domain family 4 member J; Immunoglobulin E-binding factor; Lymphocyte IgE receptor

Recovery

Matrices listed below were spiked with certain level of recombinant FceRII and the recovery rates were calculated by comparing the measured value to the expected amount of FceRII in samples.

Matrix	Recovery range	Average
serum(n=5)	90-104%	97%
EDTA plasma(n=5)	80-97%	88%
Heparin plasma(n=5)	87-99%	93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FceRII and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-97%	83-96%	95-103%	88-95%
EDTA plasma(n=5)	86-93%	88-92%	86-97%	90-101%
Heparin plasma(n=5)	91-99%	85-93%	82-95%	93-99%

Typical Data

<table> <tbody><tr> <th>Concentration (pg/mL)</th> <th>OD</th> <th>Corrected OD</th> </tr> <tr> <td>100.00</td> <td>2.121</td> <td>2.025</td> </tr> <tr> <td>50.00</td> <td>1.755</td> <td>1.659</td> </tr> <tr> <td>25.00</td> <td>1.225</td> <td>1.129</td> </tr> <tr> <td>12.50</td> <td>0.847</td> <td>0.751</td> </tr> <tr> <td>6.25</td> <td>0.525</td> <td>0.429</td> </tr> <tr> <td>3.13</td> <td>0.352</td> <td>0.256</td> </tr> <tr> <td>1.57</td> <td>0.208</td> <td>0.112</td> </tr> <tr> <td>0.00</td> <td>0.096</td> <td>0.000</td> </tr> </tbody></table>

Limit Of Detection

100 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FceRII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FceRII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FceRII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FceRII in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

<a href="https://s3.us-west-1.amazonaws.com/s3.biohippo.com/elisakits/elk/ELK3208-1.pdf" target="_blank">Datasheet</a>

UniProt ID	P20693

Applications	ELISA
Research Area	CD & Adhesion molecule;Infection immunity;Rheumatology;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15206775	ELK3208	48T	$320		Add to Cart
BHE15206775	ELK3208	96T	$458		Add to Cart

Mouse FceRII(Receptor II For The Fc Region Of Immunoglobulin E) ELISA Kit

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