Mouse Fc gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) ELISA Kit PicoKine®

Mfr.No.

EK1313

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.004	0.091	0.155	0.290	0.491	0.830	1.484	2.103

Description

Mouse Fc gamma RII/RIII (CD32/CD16) (Fcgr2b/Fcgr3) ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Fcgr2b/Fcgr3 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Low affinity immunoglobulin gamma Fc region receptor II;Fc gamma receptor IIB;Fc-gamma RII;Fc-gamma-RIIB;FcRII;IgG Fc receptor II beta;Lymphocyte antigen 17;Ly-17;CD32;Fcgr2;Fcgr2b, Ly-17;

Molecular Weight

36695 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: T40-R217

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Fcgr2b/Fcgr3

Subcellular Localization

Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	89	216	932	94	236	885
Standard deviation	5.05	16.63	46.6	6.95	20.06	46.9
CV (%)	5%	7.7%	5%	7.4%	8.5%	5.3%

Protein Function

Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin- coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B- cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively.

Background

CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	89	87	98	86	90	4.74	5.2%
Sample 2	216	235	229	206	221	11.28	5.1%
Sample 3	932	935	841	848	889	44.58	5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	14130, 14131
Gene Name	FCGR2B
UniProt ID	P08101, P08508
Protein Name	Low affinity immunoglobulin gamma Fc region receptor II-b/III
Tissue Specificity	Widely expressed by cells of hemopoietic origin. The isoforms are differentially expressed. Isoform IIB1 is preferentially expressed by cells of the lymphoid lineage, isoform IIB2 by cells of the myeloid lineage, and isoform IIB3 is released by macrophages and is present in the serum. Isoform IIB1' is expressed in myeloid and lymphoid cell lines, in normal spleen cells, and in resting or LPS-activated B-cells but is not detected in mesenteric lymph node cells.
Sequence	Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000920	EK1313	96 wells/kit	$750		Add to Cart

Mouse Fc gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) ELISA Kit PicoKine®

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