Mouse Factor-related Apoptosis ligand,FASL ELISA Kit

Mfr.No.

CSB-E04545m

Alternative Names

Fas ligand (TNF superfamily, member 6), APT1LG1, CD178, CD95L, FASL, TNFSF6, CD95 ligand, apoptosis (APO-1) antigen ligand 1, fas ligand, tumor necrosis factor (ligand) superfamily, member 6

Reactivity

Mouse

Detection Range

7.8 pg/mL-500 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human TL1A/TNFSF15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	92-106
EDTA plasma (n=4)	101	95-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TL1A/TNFSF15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-93
1:2	Average %	97
	Range %	92-100
1:4	Average %	94
	Range %	86-97
1:8	Average %	100
	Range %	89-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.471	2.397	2.434	2.295
25	1.890	1.792	1.841	1.702
12.5	1.259	1.223	1.241	1.102
6.25	0.841	0.832	0.837	0.698
3.12	0.469	0.455	0.462	0.323
1.56	0.324	0.314	0.319	0.180
0.78	0.229	0.218	0.224	0.085
0	0.141	0.137	0.139	?

Species

Mus musculus

Sensitivity

1.95 pg/mL

Limit Of Detection

450 nm

UniProt ID	P41047
Antigen	Fas ligand (TNF superfamily, member 6)

Applications

ELISA

Research Area

FASLG

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505682	CSB-E04545m	96T	$595	Add to Cart
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BHE10505682	CSB-E04545m	10x96T	$5652.50	Add to Cart

Mouse Factor-related Apoptosis ligand,FASL ELISA Kit

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