Mouse factor B,BF ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E04695m

Alternative Names

factor B,BF

Reactivity

Mouse

Detection Range

0.312 ug/mL-20 ug/mL

Sample Type

Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of canine BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-105
EDTA plasma (n=4)	96	92-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of canine BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-105
1:2	Average %	91
	Range %	88-96
1:4	Average %	95
	Range %	90-102
1:8	Average %	97
	Range %	92-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

0.078 ug/mL

Limit Of Detection

450 nm

UniProt ID	P04186
Antigen	factor B,BF

Applications

ELISA

Research Area

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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