Mouse Estrogen Receptor Beta - ER-beta ELISA Kit

Mfr.No.

E1412Mo

Sensitivity

0.12ng/ml

Detection Range

0.3-90ng/ml

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Mouse Nesfatin1 (also known as ER-beta) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

ER-beta;ESR 2;ESR2;Estrogen receptor beta;Nuclear receptor subfamily 3 group A member 2

Molecular Weight

59070

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1412Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	11.1	0.59	5.3
Intra-Assay	2	18	26.3	0.79	3
Intra-Assay	3	18	6.5	0.4	6.1

Background

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1 (ER-alpha), and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. May play a role in ovarian follicular growth and maturation.

Protocol

These standard curves of E1412Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.33	2.341	2.304
	2.352
24ng/ml	1.518	1.508	1.471
	1.498
12ng/ml	0.743	0.84	0.803
	0.937
6ng/ml	0.527	0.533	0.496
	0.54
3ng/ml	0.237	0.267	0.23
	0.297
0ng/ml	0.038	0.037	0
	0.035

Gene ID	13983
UniProt ID	O08537

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109275	E1412Mo	48T	$320		Add to Cart
BHE12109275	E1412Mo	96T	$458		Add to Cart

Mouse Estrogen Receptor Beta - ER-beta ELISA Kit

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