Mouse estrogen,E ELISA Kit

Mfr.No.

CSB-E07280m

Reactivity

Mouse

Detection Range

25 pg/mL-1000 pg/mL

Sample Type

Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human ASPN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	94-104
EDTA plasma (n=4)	92	85-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ASPN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	102
	Range %	93-108
1:2	Average %	97
	Range %	89-101
1:4	Average %	93
	Range %	88-97
1:8	Average %	99
	Range %	90-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

25 pg/mL

Limit Of Detection

450 nm

Antigen	estrogen,E

Applications

ELISA

Research Area

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Bisphenol A Initiates Excessive Premature Activation of Primordial Follicles in Mouse Ovaries via the PTEN Signaling Pathway Hu Y.et al,Reprod Sci.,Oct 5

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