Mouse Estradiol 17-beta-dehydrogenase 2

Mfr.No.

E1693Mo

Sensitivity

0.043ng/ml

Detection Range

0.1-35ng/ml

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Mouse Estradiol 17-Beta-Dehydrogenase 2 (also known as HSD17B2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

17-beta-HSD 2;17-beta-hydroxysteroid dehydrogenase type 2;Estradiol 17-beta-dehydrogenase 2;HSD 17B2;HSD17B2;Testosterone 17-beta-dehydrogenase

Molecular Weight

41836

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1693Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.2	0.18	3.4
Intra-Assay	2	18	15.8	0.45	2.8
Intra-Assay	3	18	11.1	0.48	4.3

Background

Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.

Protocol

These standard curves of E1693Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	1.813	1.914	1.88
	2.016
10ng/ml	1.101	0.988	0.954
	0.874
5ng/ml	0.42	0.429	0.395
	0.438
2.5ng/ml	0.169	0.169	0.135
	0.17
1.25ng/ml	0.09	0.106	0.072
	0.122
0ng/ml	0.026	0.034	0
	0.042

Gene ID	15486
UniProt ID	P51658

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109526	E1693Mo	48T	$320		Add to Cart
BHE12109526	E1693Mo	96T	$458		Add to Cart

Mouse Estradiol 17-beta-dehydrogenase 2 - HSD17B2 ELISA Kit

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