Mouse Epinephrine/Adrenaline,EPI ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08679m

Sensitivity

0.125 ng/mL

Detection Range

0.25 ng/mL-50 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Epinephrine/Adrenaline,EPI

Recovery

The recovery of human ASS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	99	95-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ASS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-97
1:2	Average %	97
	Range %	92-105
1:4	Average %	85
	Range %	81-88
1:8	Average %	89
	Range %	83-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	EPI

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Intestinal gluconeogenesis is crucial to maintain a physiological fasting glycemia in the absence of hepatic glucose production in mice Penhoat A et al,Metabolism,2013 Oct 14.pii: S0026-0495(13)00289-8. Activation of RAW 264.7 cells by a polysaccharide isolated from Antarctic bacterium Pseudoaltermonas sp. S-5 Jing Li, et al,Carbohydrate Polymers,2015 Oct 5;130:97-103 AMP-Activated Protein Kinase (AMPK) Regulates Energy Metabolism through Modulating Thermogenesis in Adipose Tissue Lingyan Wu.et al,ORIGINAL RESEARCH ARTICLE,Feb 21

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