Mouse Epidermal growth factor-like protein 6(EGFL6) ELISA kit

Mouse Epidermal growth factor-like protein 6(EGFL6) ELISA kit

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Mfr.No.

CSB-EL007475MO

Sensitivity

23.43 pg/mL

Detection Range

93.75 pg/mL-6000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

EGF-like-domain, multiple 6, DKFZp564P2063, MAEG, W80, EGF repeat-containing protein 6, MAM and EGF domain containing, epidermal growth factor-like protein 6

Recovery

The recovery of Human NF-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-90
EDTA plasma (n=4)	94	88-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of Human NF-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-98
1:2	Average %	97
	Range %	93-101
1:4	Average %	93
	Range %	84-97
1:8	Average %	103
	Range %	95-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.513	2.614	2.564	2.396
20	2.017	2.115	2.066	1.898
10	1.442	1.343	1.393	1.225
5	0.887	0.864	0.876	0.708
2.5	0.531	0.545	0.538	0.370
1.25	0.374	0.362	0.368	0.200
0.625	0.276	0.284	0.280	0.112
0	0.167	0.168	0.168

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9JJZ5

Applications	ELISA
Research Area	EGFL6

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505657	CSB-EL007475MO	96T	$695	Add to Cart
BHE10505657	CSB-EL007475MO	5x96T	$3336	Add to Cart
BHE10505657	CSB-EL007475MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.