Mouse Eotaxin 2 ELISA Kit

Mfr.No.

CSB-E07278m

Sensitivity

3.9 pg/ml

Detection Range

15.6 pg/ml - 1000 pg/ml

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

chemokine (C-C motif) ligand 24, Ckb-6, MPIF-2, MPIF2, SCYA24, CK-beta-6, OTTHUMP00000210225, eotaxin-2, myeloid progenitor inhibitory factor 2, small inducible cytokine A24, small inducible cytokine subfamily A (Cys-Cys), member 24

Recovery

The recovery of pig FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-103
EDTA plasma (n=4)	96	93-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-99
1:2	Average %	95
	Range %	87-106
1:4	Average %	96
	Range %	90-110
1:8	Average %	91
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pmol/L	OD1	OD2	Average	?
4	2.771	2.736	2.754	?
8	1.459	1.442	1.451	?
16	0.827	0.865	0.846	?
32	0.575	0.565	0.570	?
64	0.318	0.307	0.313	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q9JKC0

Applications	ELISA
Research Area	CCL24

Storage	Store at 2-8°C. Please refer to protocol.

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Mouse Eotaxin 2 ELISA Kit

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