Mouse Eotaxin-1 ELISA Kit PicoKine®

Mfr.No.

EK0330

Sensitivity

<1 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.064	0.193	0.331	0.525	0.990	1.375	1.678	1.952

Description

Mouse Eotaxin-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl11 in cell culture supeatants, serum and plasma (citrate, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Eotaxin;C-C motif chemokine 11;Eosinophil chemotactic protein;Small-inducible cytokine A11;Ccl11;Scya11;

Molecular Weight

10893 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H24-P97

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Eotaxin

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

citrate or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	34	223	405	33	223	429
Standard deviation	1.49	11.59	26.4%2	2.07	12.71	33.46
CV (%)	4.4%	5.2%	6.4%	5.7%%	5.7%	7.8%

Protein Function

In response to the presence of allergens, this protein ly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils.

Background

Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	34	36	38	36	36	1.41	3.9%
Sample 2	223	227	217	219	221	3.84	1.7%
Sample 3	405	412	415	422	413	6.1	1.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	20292
Gene Name	CCL11
UniProt ID	P48298
Protein Name	Eotaxin
Tissue Specificity	Expressed constitutively in the thymus. Expression inducible in the lung (type I alveolar epithelial cells), intestine, heart, spleen, kidney.

Applications	ELISA
Research Area	Chemokines,Immunology,Innate Immunity

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000357	EK0330	96 wells/kit	$499		Add to Cart

Mouse Eotaxin-1 ELISA Kit PicoKine®

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