Mouse ENTPD2 ELISA Kit PicoKine®

Mfr.No.

EK2014

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.036	0.068	0.126	0.207	0.344	0.588	1.047	1.942

Description

Mouse ENTPD2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Entpd2 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Ectonucleoside triphosphate diphosphohydrolase 2; NTPDase 2; CD39 antigen-like 1; Ecto-ATP diphosphohydrolase 2; Ecto-ATPDase 2; Ecto-ATPase 2; Entpd2; Cd39l1

Immunogen

Expression system for standard: NS0; Immunogen sequence: C26-S462

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse ENTPD2

Subcellular Localization

Cell membrane. Multi-pass membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	22	91	412	28	113	431
Standard deviation	1.06	4.73	22.25	1.43	6.22	25.86
CV (%)	4.8%	5.2%	5.4%	5.1%	5.5%	6.0%

Protein Function

In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Hydrolyzes ADP only to a marginal extent.

Background

Ectonucleoside triphosphate diphosphohydrolase 2 is an enzyme that in humans is encoded by the ENTPD2 gene. This gene is mapped to 2; 2 A3. The protein encoded by this gene is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5'-triphosphates. This ecto-ATPase is an integral membrane protein. Alteative splicing of this gene results in multiple transcript variants. It has been shown, by scientists from the University of Warwick, that E-NTPDase2 stimulates the growth of the eye: by testing the enzyme on tadpoles, the tadpoles were found to develop extra eyes on their body.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	22	23	22	23	22	0.50	2.2%
Sample 2	91	104	95	96	96	4.71	4.9%
Sample 3	412	477	456	451	449	23.48	5.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	12496
Gene Name	Entpd2
UniProt ID	O55026
Protein Name	Ectonucleoside triphosphate diphosphohydrolase 2

Applications	ELISA
Research Area	Cancer,Metabolism,Neurology Process,Neuroscience

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001550	EK2014	96 wells/kit	$750		Add to Cart

Mouse ENTPD2 ELISA Kit PicoKine®

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