Matrices listed below were spiked with certain level of Enpep and the recovery rates were calculated by comparing the measured value to the expected amount of Enpep in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-103
98
EDTA plasma(n=5)
87-97
94
heparin plasma(n=5)
85-103
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Enpep and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-96%
94-101%
86-105%
EDTA plasma(n=5)
82-100%
89-99%
84-100%
heparin plasma(n=5)
82-90%
85-100%
88-99%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
P16406
Applications
ELISA
Applications Notes
Enpep ELISA Kit allows for the in vitro quantitative determination of Enpep concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Enpep (Glutamyl aminopeptidase) ELISA Kit