Mouse Endothelial nitric oxide synthase,eNOS ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08324m

Alternative Names

nitric oxide synthase 3 (endothelial cell), ECNOS, eNOS, nitric oxide synthase 3

Reactivity

Mouse

Detection Range

0.312 IU/mL-20 IU/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of guinea pig IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	92-97
EDTA plasma (n=4)	92	84-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-96
1:2	Average %	94
	Range %	89-99
1:4	Average %	103
	Range %	98-108
1:8	Average %	100
	Range %	91-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

0.078 IU/mL

Limit Of Detection

450 nm

UniProt ID	P70313
Antigen	nitric oxide synthase 3 (endothelial cell)

Applications

ELISA

Research Area

NOS3

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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