Mouse Endosialin(CD248) ELISA kit

Mouse Endosialin(CD248) ELISA kit

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Mfr.No.

CSB-EL004905MO

Alternative Names

CD248 molecule, endosialin, CD164L1, MGC119478, MGC119479, TEM1, 2610111G01Rik, CD164 sialomucin-like 1, CD248 antigen, endosialin, endosialin, tumor endothelial marker 1

Reactivity

Mouse

Detection Range

7.81 pg/mL-500 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human PARP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	97-105
EDTA plasma (n=4)	92	86-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PARP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	92
	Range %	87-96
1:4	Average %	100
	Range %	97-103
1:8	Average %	95
	Range %	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.301	2.369	2.335	2.188
500	1.582	1.598	1.590	1.443
250	0.948	0.968	0.958	0.811
125	0.578	0.585	0.582	0.435
62.5	0.389	0.368	0.379	0.232
31.25	0.287	0.264	0.276	0.129
15.6	0.205	0.213	0.209	0.062
0	0.147	0.146	0.147

Species

Mus musculus

Sensitivity

1.95 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q91V98
Antigen	CD248 molecule, endosialin

Applications

ELISA

Research Area

CD248

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505632	CSB-EL004905MO	96T	$695	Add to Cart
BHE10505632	CSB-EL004905MO	5x96T	$3336	Add to Cart
BHE10505632	CSB-EL004905MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.