Matrices listed below were spiked with certain level of EL and the recovery rates were calculated by comparing the measured value to the expected amount of EL in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-101
93
EDTA plasma(n=5)
86-103
96
heparin plasma(n=5)
85-100
90
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.