Mouse EGF-like repeat and discoidin I-like domain-containing protein 3(EDIL3) ELISA kit

Mfr.No.

CSB-EL007399MO

Alternative Names

EGF-like repeats and discoidin I-like domains 3, DEL1, MGC26287, EGF-like repeats and discoidin I-like domains-containing protein 3, developmental endothelial locus-1

Reactivity

Mouse

Detection Range

28 pg/mL-1800 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human NAPSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	95-104
EDTA plasma (n=4)	101	94-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human NAPSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	104
	Range %	97-108
1:10	Average %	101
	Range %	95-104
1:20	Average %	97
	Range %	89-101
1:40	Average %	106
	Range %	98-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
300	2.435	2.527	2.481	2.289
150	2.042	2.104	2.073	1.881
75	1.455	1.409	1.432	1.240
37.5	0.843	0.823	0.833	0.641
18.75	0.552	0.538	0.545	0.353
9.4	0.381	0.376	0.379	0.187
4.7	0.301	0.311	0.306	0.114
0	0.190	0.194	0.192	?

Species

Mus musculus

Sensitivity

7 pg/mL

Limit Of Detection

450 nm

UniProt ID	O35474
Antigen	EGF-like repeats and discoidin I-like domains 3

Applications

ELISA

Research Area

EDIL3

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505624	CSB-EL007399MO	96T	$695	Add to Cart
BHE10505624	CSB-EL007399MO	5x96T	$3336	Add to Cart
BHE10505624	CSB-EL007399MO	10x96T	$6602.50	Add to Cart

Mouse EGF-like repeat and discoidin I-like domain-containing protein 3(EDIL3) ELISA kit

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