Matrices listed below were spiked with certain level of Efna4 and the recovery rates were calculated by comparing the measured value to the expected amount of Efna4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-100
96
EDTA plasma(n=5)
92-100
96
heparin plasma(n=5)
90-105
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Efna4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-97%
93-103%
90-100%
EDTA plasma(n=5)
91-97%
94-99%
96-100%
heparin plasma(n=5)
91-99%
90-98%
94-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
O08542
Applications
ELISA
Applications Notes
Efna4 ELISA Kit allows for the in vitro quantitative determination of Efna4 concentrations in serum, plasma, tissue homogenates and other biological fluids.