Mouse Ectonucleotide pyrophosphatase/phosphodiesterase family member 1(ENPP1) ELISA kit

Mouse Ectonucleotide pyrophosphatase/phosphodiesterase family member 1(ENPP1) ELISA kit

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Mfr.No.

CSB-EL007679MO

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

ectonucleotide pyrophosphatase/phosphodiesterase 1, M6S1, NPP1, NPPS, PC-1, PCA1, PDNP1, Ly-41 antigen, alkaline phosphodiesterase 1, membrane component, chromosome 6, surface marker 1, phosphodiesterase I/nucleotide pyrophosphatase 1, plasma-cell membra

Recovery

The recovery of sheep NGAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-97
EDTA plasma (n=4)	95	88-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep NGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:200	Average %	92
	Range %	86-95
1:400	Average %	103
	Range %	99-105
1:800	Average %	94
	Range %	88-98
1:1600	Average %	99
	Range %	94-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1000	0.115	0.118	0.117
500	0.227	0.231	0.229
250	0.483	0.504	0.494
125	0.956	1.039	0.998
62.5	1.425	1.438	1.432
31.25	2.354	2.366	2.360
15.6	2.652	2.671	2.662
0	2.927	2.945	2.936

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P06802

Applications	ELISA
Research Area	ENPP1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10505619	CSB-EL007679MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.