Matrices listed below were spiked with certain level of DNMT1 and the recovery rates were calculated by comparing the measured value to the expected amount of DNMT1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
95-99
98
EDTA plasma(n=5)
86-102
96
heparin plasma(n=5)
85-105
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DNMT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-95%
88-103%
85-105%
EDTA plasma(n=5)
83-101%
85-93%
82-100%
heparin plasma(n=5)
80-97%
80-100%
82-97%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P13864
Applications
ELISA
Applications Notes
DNMT1-methyltransferase 1) ELISA Kit allows for the in vitro quantitative determination of DNMT1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse DNMT1(DNA(cytosine-5)-methyltransferase 1) ELISA Kit