Mouse Disintegrin and metalloproteinase domain-containing protein 33(ADAM33) ELISA kit

Mouse Disintegrin and metalloproteinase domain-containing protein 33(ADAM33) ELISA kit

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Mfr.No.

CSB-EL001290MO

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

ADAM metallopeptidase domain 33, RP5-964F7.2, C20orf153, DJ964F7.1, DKFZp434K0521, FLJ35308, FLJ36751, MGC149823, MGC71889, a disintegrin and metalloprotease 33, a disintegrin and metalloproteinase domain 33, disintegrin and reprolys

Recovery

The recovery of mouse VEGF-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	96	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGF-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	95
	Range %	91-105
1:4	Average %	102
	Range %	92-110
1:8	Average %	90
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.600	2.511	2.556	2.462
50	2.020	2.152	2.086	1.992
25	1.391	1.366	1.379	1.285
12.5	0.735	0.744	0.740	0.646
6.25	0.331	0.321	0.326	0.232
3.12	0.255	0.235	0.245	0.151
1.56	0.188	0.186	0.187	0.093
0	0.097	0.091	0.094	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q923W9

Applications	ELISA
Research Area	ADAM33

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505600	CSB-EL001290MO	96T	$595	Add to Cart
BHE10505600	CSB-EL001290MO	5x96T	$2856	Add to Cart
BHE10505600	CSB-EL001290MO	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.