Mouse Dihydrotestosterone,DHT ELISA Kit

Mouse Dihydrotestosterone,DHT ELISA Kit

Reviews of Mouse Dihydrotestosterone,DHT ELISA Kit

Rate it now..!!

Rate it now..!!

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Mfr.No.

CSB-E07880m

Alternative Names

Dihydrotestosterone,DHT

Reactivity

Mouse

Detection Range

0.1 nmol/L-20 nmol/L

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human ASP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	100-110
EDTA plasma (n=4)	90	85-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ASP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	97
	Range %	91-103
1:200	Average %	89
	Range %	84-95
1:400	Average %	95
	Range %	90-99
1:800	Average %	84
	Range %	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.527	2.452	2.490	2.390
5	1.858	1.976	1.917	1.817
2.5	1.204	1.294	1.249	1.149
1.25	0.657	0.616	0.637	0.537
0.625	0.356	0.377	0.367	0.267
0.312	0.201	0.211	0.206	0.106
0.156	0.134	0.129	0.132	0.032
0	0.098	0.101	0.100

Species

Mus musculus

Sensitivity

0.05 nmol/L

Limit Of Detection

450 nm

Antigen	Dihydrotestosterone,DHT

Applications

ELISA

Research Area

DHT

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Cyp3a deficiency enhances androgen receptor activity and cholesterol synthesis in the mouse prostate. Hashimoto M.et al,J Steroid Biochem Mol Biol.,(16)30126-1

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10505582	CSB-E07880m	96T	$835	Add to Cart
BHE10505582	CSB-E07880m	5x96T	$4008	Add to Cart
BHE10505582	CSB-E07880m	10x96T	$7932.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.