Mouse Dihydrotestosterone,DHT ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E07880m

Sensitivity

0.05 nmol/L

Detection Range

0.1 nmol/L-20 nmol/L

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Dihydrotestosterone,DHT

Recovery

The recovery of human ASP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	100-110
EDTA plasma (n=4)	90	85-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ASP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	97
	Range %	91-103
1:200	Average %	89
	Range %	84-95
1:400	Average %	95
	Range %	90-99
1:800	Average %	84
	Range %	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	DHT

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Cyp3a deficiency enhances androgen receptor activity and cholesterol synthesis in the mouse prostate. Hashimoto M.et al,J Steroid Biochem Mol Biol.,(16)30126-1

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