Mouse Dickkopf-related protein 1(DKK1) ELISA kit

Mfr.No.

CSB-EL006920MO

Sensitivity

13.127 pg/mL

Detection Range

78.125 pg/mL-5000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

dickkopf homolog 1 (Xenopus laevis), DKK-1, SK, dickkopf homolog 1, dickkopf related protein-1, dickkopf-1 like

Recovery

The recovery of goat VB12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of goat VB12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:10	Average %	85
	Range %	80-92
1:20	Average %	97
	Range %	90-105
1:40	Average %	97
	Range %	92-104
1:80	Average %	92
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	O54908

Applications	ELISA
Research Area	DKK1

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10505576	CSB-EL006920MO	10x96T	$3562.50	Add to Cart