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Home / ELISA Kit

Mouse Diablo homolog, mitochondrial (DIABLO) ELISA Kit

SKU : BHE13706443

DIABLO encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in DIABLO is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.

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Mfr.No.	KTE71285
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Diablo homolog, mitochondrial (DIABLO) ELISA Kit employs a two-site sandwich ELISA to quantitate DIABLO.
Alternative Names	DIABLO; hCG_1782202; DIABLO-S; FLJ10537; FLJ25049; SMAC; SMAC3; 0610041G12Rik; diablo; direct IAP-binding protein with low pI; mitochondrial Smac protein; second mitochondria-derived activator of caspase
Detection Method	Colorimetric
Kit Components	Mouse Diablo homolog, mitochondrial microplate Mouse Diablo homolog, mitochondrial standard Mouse Diablo homolog, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Diablo homolog, mitochondrial (DIABLO) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DIABLO. No significant cross-reactivity or interference between Mouse DIABLO and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	DIABLO encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in DIABLO is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.
Notes	This Mouse Diablo homolog, mitochondrial (DIABLO) ELISA Kit employs a two-site sandwich ELISA to quantitate DIABLO in samples. An antibody specific for DIABLO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDIABLO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIABLO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIABLO bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	66593

Applications	ELISA
Applications Notes	This Mouse Diablo homolog, mitochondrial (DIABLO) ELISA Kit employs a two-site sandwich ELISA to quantitate DIABLO in samples. An antibody specific for DIABLO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDIABLO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIABLO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIABLO bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13706443	KTE71285	48 T	$339		Add to Cart
BHE13706443	KTE71285	96 T	$589		Add to Cart
BHE13706443	KTE71285	96 Tx5	$2629		Add to Cart
BHE13706443	KTE71285	96 Tx50	$24569		Add to Cart

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