Mouse Desmosine ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14196m

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Desmosine

Recovery

The recovery of pig C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-104
EDTA plasma (n=4)	92	87-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig C-Peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	88-98
1:2	Average %	96
	Range %	91-102
1:4	Average %	103
	Range %	97-107
1:8	Average %	87
	Range %	81-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	Desmosine

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Localization and stretch-dependence of lung elastase activity in development and compensatory growth. Young SM et al,J Appl Physiol ,2015 Apr 1;118(7):921-31. Recombinant Human Elafin Promotes Alveologenesis in Newborn Mice Exposed to Chronic Hyperoxia Han W.et al,Int J Biochem Cell Biol.,Aug 9 Elastin receptor (S-gal) occupancy by elastin peptides modulates T cell response during murine emphysema Meghraoui-Kheddar A.et al,Am J Physiol Lung Cell Mol Physiol. ,Jun 1

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