Mouse Delta-1-pyrroline-5-carboxylate Dehydrogenase

Mfr.No.

E1585Mo

Sensitivity

5.18ng/L

Detection Range

10-3000ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial (also known as ALDH4A1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

A930035F14Rik;Ahd;Ahd-;Ahd1;ALD;Aldehyde dehydrogenase family 4 member A1;Aldh4;ALDH4A1;Aldh5a1;Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial;E330022C09;L-glutamate gamma-semialdehyde dehydrogenase;P5C dehydrogenase;P5cd;P5cdh;P5cdhl;P5cdhs;Ssd;Ssdh1

Molecular Weight

61841

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1585Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	470.98	25.4	5.39
Intra-Assay	2	18	845.29	31.07	3.68
Intra-Assay	3	18	283.13	20.09	7.1

Background

Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes (By similarity).

Protocol

These standard curves of E1585Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.158	2.197	2.147
	2.235
800ng/L	1.323	1.355	1.306
	1.388
400ng/L	0.711	0.774	0.725
	0.837
200ng/L	0.505	0.515	0.466
	0.525
100ng/L	0.259	0.256	0.207
	0.253
0ng/L	0.043	0.049	0
	0.055

Gene ID	212647
UniProt ID	Q8CHT0

Research Area	Tags & Cell Markers

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109428	E1585Mo	48T	$320		Add to Cart
BHE12109428	E1585Mo	96T	$458		Add to Cart

Mouse Delta-1-pyrroline-5-carboxylate Dehydrogenase - ALDH4A1 ELISA Kit

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