Mouse Dedicator of Cytokinesis Protein 1

Mfr.No.

E1998Mo

Sensitivity

14.13ng/L

Detection Range

30-9000ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

180 kDa protein downstream of CRK;Dedicator of cytokinesis protein 1;DOCK 1;DOCK1;DOCK180

Molecular Weight

215085

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E1998Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1063.9	68.75	6.5
Intra-Assay	2	18	2025.8	114.18	5.6
Intra-Assay	3	18	481.7	35.31	7.3

Background

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.

Protocol

These standard curves of E1998Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.069	2.082	2.037
	2.095
2400ng/L	1.54	1.594	1.55
	1.648
1200ng/L	1.004	1.07	1.025
	1.135
600ng/L	0.676	0.695	0.651
	0.715
300ng/L	0.462	0.461	0.416
	0.459
0ng/L	0.06	0.045	0
	0.029

Gene ID	330662
UniProt ID	Q8BUR4

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109810	E1998Mo	48T	$320		Add to Cart
BHE12109810	E1998Mo	96T	$458		Add to Cart

Mouse Dedicator of Cytokinesis Protein 1 - DOCK1 ELISA Kit

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