Mouse Decorin ELISA Kit PicoKine®

Mfr.No.

EK0750

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.054	0.101	0.146	0.221	0.382	0.804	1.401	2.073

Description

Mouse Decorin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Dcn in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Decorin;Bone proteoglycan II;PG-S2;PG40;Dcn;

Molecular Weight

39809 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: D31-K354

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Decorin

Subcellular Localization

Secreted, extracellular space, extracellular matrix.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	68	227	930	61	239	956
Standard deviation	3.12	10.44	68.82	3.17	14.1	76.48
CV (%)	4.6%	4.6%	7.4%	5.2%	5.9%	8%

Protein Function

May affect the rate of fibrils formation.

Background

Decorin is a proteoglycan on average 90 - 140 kilodaltons (kD) in size.It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS).Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	68	67	60	65	65	3.08	4.7%
Sample 2	227	213	209	242	222	12.96	5.8%
Sample 3	930	785	877	787	844	61.66	7.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	13179
Gene Name	DCN
UniProt ID	P28654
Protein Name	Decorin
Sequence	Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

Applications	ELISA
Research Area	Collagen,Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Growth Factors/Hormones,Signal Transduction,Structures

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000602	EK0750	96 wells/kit	$499		Add to Cart

Mouse Decorin ELISA Kit PicoKine®

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