Mouse DcTRAILR2/TNFRSF22 ELISA Kit PicoKine®

Mfr.No.

EK2007

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.016	0.036	0.082	0.161	0.337	0.734	1.568	2.544

Description

Mouse DcTRAILR2/TNFRSF22 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfrsf22 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 22; Decoy TRAIL receptor 2; TNF receptor family member SOBa; TNF receptor homolog 2; Tumor necrosis factor receptor p60 homolog 2; Tnfrsf22; Dctrailr2; Tnfrh2; Tnfrsf1al2

Immunogen

Expression system for standard: NS0; Immunogen sequence: E42-R198

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TNFRSF22

Subcellular Localization

Cell membrane. Single-pass type II membrane protein. Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	29	102	469	31	116	475
Standard deviation	1.42	5.41	25.33	2.05	7.89	33.25
CV (%)	4.9%	5.3%	5.4%	6.6%	6.8%	7.0%

Protein Function

Receptor for the cytotoxic ligand TNFSF10/TRAIL. Lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. Protects cells against TRAIL mediated apoptosis possibly through ligand competition. Cannot induce the NF-kappa-B pathway.

Background

TNFRSF22 interacts via a complex receptor system to preferentially induce apoptosis of tumor cells in vivo and in vitro. This gene is mapped to 7 F5; 7 88.27 cM. The mouse decoy TRAIL R2 (TNFRSF22) gene encodes a 198 amino acid (aa) residues type I transmembrane protein with a putative 39 aa signal peptide, a 136 aa extracellular domain, a 18 aa transmembrane region, and a 5 aa cytoplasmic tail. An alteatively spliced variant encoding a secreted soluble receptor of 180 aa has also been described. The transmembrane and soluble receptors are known as mDcTRAIL R2L and mDcTRAIL R2S, respectively. Both mDcTRAIL R2 isoforms and the GPI-linked mDcTRAIL R1 bind TRAIL specifically but are incapable of triggering signal transduction. Mouse DcTRAIL R1 and mDcTRAIL R2 share 71% sequence identity. The two receptors have similar structural modules in their CRDs, which differ from those found in the CRDs of mTNF RH3. Mouse DcTRAIL R1 preferentially binds mTRAIL while mDcTRAIL R2 binds both human and mTRAIL. mRNA of mouse DcTRAIL R1 and -2 are expressed at low levels in many mouse tissues and at higher levels in several mouse cell lines.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	29	34	31	32	31	1.80	5.8%
Sample 2	102	105	105	112	106	3.67	3.4%
Sample 3	469	508	495	507	494	15.72	3.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	79202
Gene Name	Tnfrsf22
UniProt ID	Q9ER62
Protein Name	Tumor necrosis factor receptor superfamily member 22
Tissue Specificity	Ubiquitous.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001542	EK2007	96 wells/kit	$750		Add to Cart

Mouse DcTRAILR2/TNFRSF22 ELISA Kit PicoKine®

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