Matrices listed below were spiked with certain level of Dapk1 and the recovery rates were calculated by comparing the measured value to the expected amount of Dapk1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-104
99
EDTA plasma(n=5)
85-101
90
heparin plasma(n=5)
87-103
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Dapk1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-101%
89-101%
89-105%
EDTA plasma(n=5)
85-100%
82-98%
84-100%
heparin plasma(n=5)
81-99%
89-99%
84-96%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q80YE7
Antigen
Dapk1
Applications
ELISA
Applications Notes
Dapk1 ELISA Kit allows for the in vitro quantitative determination of Dapk1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Dapk1(Death-associated protein kinase 1) ELISA Kit